一般演題

一覧　トップ

膵臓癌における12q21上の共通欠失領域の解析: エミール M. ヨセフ¹,古川　徹¹,八岡利昌^{1, 2},福重真一¹,砂村真琴²,小針雅男²,松野正紀²,堀井 明¹（東北大・医・¹分子病理、²一外）

Identification and characterization of the frequently deleted region in 12q21 in human pancreatic cancer: Emile M. YOUSSEF¹, Toru FURUKAWA¹, Toshimasa YATSUOKA^1,2, Shinichi FUKUSHIGE¹, Makoto SUNAMURA², Masao KOBARI², Seiki MATSUNO², and Akira HORII¹ (Depts. ¹Mol. Pathol. and ²Surg. I, Tohoku Univ. Med. Sch.)

In an effort to identify a gene involved in the development and/or progression of human pancreatic adenocarcinoma, we surveyed allelic loss in all autosomes and identified frequent loss (>30%) in chromosome arms 1p, 6q, 9p, 12q, 17p, and 18q. Further analysis for 12q revealed frequent deletions on two regions: one on 12q21 and the other on 12q22-q23.1. In this study, we further focused on 12q21, the 1-cM region between D12S81 and D12S1719, because this was the most frequently lost region in 12q. In order to identify a putative tumor suppressor gene, a contig consisting of YACs and BACs to cover the deleted region was constructed. The region was covered by 6 YAC clones which overlapped each other. FISH analysis employing subcloned cosmid clones harboring microsatellite markers in the region of interest revealed frequent loss in cultured pancreatic cancer cells. An analysis for restriction-enzyme mapping suggested a possible existence of the CpG island in this region. The physical map constructed in this study provides an ideal resource for cDNA isolation and EST mapping. Intensive searching for ESTs gave some candidate genes to be investigated. Whole sequencing of the region is in work as well.