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ヒト第１６染色体短腕に位置する新規p53標的遺伝子の単離：呉晶晶¹,小山公美子¹,岡村修¹,三好康雄¹,中村祐輔^1,2（¹阪大・医・バイオセ・臨床遺伝, ²東大・医科研・ヒトゲノムセ・シークエンス解析分野）

Isolation and characterization of a novel wild-type p53-inducible gene on human choromosome 16p11.2:Ching Ching NG¹,Kumiko KOYAMA¹,Shu OKAMURA¹,Yasuo MIYOSHI¹,Yusuke NAKAMURA^1,2 (¹Dept. Med. Genet.,Med. Dept.,Biomed. Res. Center,Osaka Univ., ²Lab. Mol. Med.,Inst. Med. Sci.,Hum. Genome Center,Tokyo Univ.)

p53, a tumour suppressor gene plays a significant role in cell cycle arrest and apoptosis, but its signaling pathways are not fully understood. In this study, we performed differential display analysis to isolate genes regulated by wild-type p53 from a colon cancer cell-line carrying a highly regulated wild type p53 expression system. We report here identification of a novel wild-type p53 inducible gene that was mapped on chromosome 16p11.2. Multiple-tissue northern blot analysis showed expression of a 1.8-kb transcript predominantly in the testis. Since we have also found a presence of a p53-binding site in this gene by genomic DNA sequences, it is certain that this gene is the novel p53-inducible gene although its biological role remains unclear.