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Crk癌化細胞のMMP-2の活性化はc-Rasを必要とする： 劉　恩波¹, タン エイエイ², 吉川史隆¹, 濱口道成² (名大¹　産婦、²病態研　分子病態）
　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　
MMP-2 activition in crk-transfected fibroblast is c-Ras dependent ： Enbo LIU¹, Aye Aye THANT², Fumitaka KIKKAWA¹, Michinari HAMAGUCHI² ( ¹Dept. Obstet Gynecol, ²Res. Inst. Dis. Mech. &Cont., Nagoya Univ. Sch. Med.)

To study the signaling pathway critical for tumor invasion, we examined the effects of dominant negative ras ( S17N ras ) expression on the production and activition of MMP-2 in crk-transfected 3Y1 cells. MMP-2 is known to be a zinc-containing endo-proteinase that is responsible for proteolytic degradation of specific extracellular matrix ( ECM ) components, and plays a key role in cancer invasion and metastasis. v-Crk overexpressing 3Y1 cells were stably transfected with a dominant negative ras ( S17Nras ) under the control of conditionally inducible MMTV promoter. In crk-transfected 3Y1 cells, expression of S17N Ras did not suppress either Crk expression or tyrosine phosphorylation of cellular proteins, but strongly inhibited the augmented secretion and proteolytic activition of MMP-2. Thus these results strongly suggest that c-Ras signaling is required for the activition of MMP-2 in crk-transfected 3Y1 cells.