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FISH による胃癌進展過程における染色体セントロメアの不安定性の解析 : 北山康彦¹、甲田賢治¹、椙村春彦² ( ¹ 藤枝市立総合病院病理、² 浜松医科大学病理学第一 )

Analysis of chromosomal centromeric instability during gastric cancer development by FISH : Yasuhiko KITAYAMA¹, Kenji KODA¹, Haruhiko SUGIMURA² ( ¹ Dept. Pathol. Fujieda Municipal General Hosp., ² 1 st Dept. Pathol. Hamamatsu Univ. Sch. Med )

The process of tumorgenesis is driven by the progressive accumulation of chromosomal aberrations. Numerical chromosome aberration in gastric cancer were investigated using a panel of 17 chromosome-specific centromeric α-satellite DNA probes except cocktail ones ( chrom 5, 9, 13, 14, 19, 21 and 22). Fresh touch preparations of 31 surgically resected gastric cancer tissues were analyzed by FISH and compared with histopathological variables. Numerical abnormalities in gastric cancer were most frequently found in chrom 1 and 2 from the early stage. Though chromosomal abnormality became extensive in a step-wise manner according to cancer progression, the abnormalities in chrom 6, 7, 10, 11 and Y occur in a relatively late phase, having a low frequency. Especially chrom 3 and 18 seems to be extremely protected and stable in terms of centromere numerical abnormality even after the tumor cells extending beyond the gastric wall. Gain was more frequently demonstrated, while the loss was seen in chrom 17, 18, 20, and Y. Furthermore, the profile of these sequential process was similar both in tubular and mucocellular type of cancer, but the prevalence were different.