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マウスPur-1遺伝子の構造と発現：古賀千恵,宋軍,横山和尚（理研筑セ）

Genomic Organization and Expression of Mouse Gene for Pur-1: Chie KOGA, Jun SONG, and Kazunari K. YOKOYAMA (Tsukuba Life Science Center, RIKEN, Japan)

We have characterized the genomic structure and the expression of the mouse gene for Pur-1. The cloned Pur-1 gene spans a 5-kb region that includes the promoter, five exons, four introns and one 3'-untranslated region. All exon-intron junction sequences conform to the GT/AG rule. The promoter region has features typical of a housekeeping gene: a high G+C content (77.5%); a high frequency of CpG dinucleotides, in particular within the region 0.5 kb upstream of the site of initiation of translation; and the absence of canonical TATA and CAAT boxes. An S1 nuclease protection assay demonstrated the presence of multiple sites for initiation of transcription around a site 108 nucleotides (nt) upstream of the ATG codon. The genomic organization and the expression patterns of Pur-1 showed the characteristics of the housekeeping gene. Sequence analysis of the 5'-flanking region also revealed numerous potential binding sites for transcription factors Sp1 and AP-2. Positive element controlling basal transcription of the human Pur-1 gene was located from nt -258 to +43 (relative to the major transcription initiation site). Thus, the gene for Pur-1 is a highly conserved mouse equivalent of human MAZ (Myc-associated zinc finger protein).