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人体腫瘍組織内オステオポンチンの特異抗体による免疫組織学的検索：前田雅弘¹, 清藤勉¹,上出利光²,鈴木利光³,丸山孝士¹（¹（株）免疫生物研究所,²北大・免疫研・免疫病態,³福島医大・第二病理）

Immunohistological analysis of osteopontin with monoclonal antibody in human tumors: Masahiro MAEDA¹,Tsutomu SEITO¹,Toshimitsu UEDE¹,Toshimitsu SUZUKI³,Koshi MARUYAMA¹ (¹Immunobiol.Lab.Co.Ltd., ²Sec.Immunopathogenesis,Inst.Immunol.Sci.,Hokkaido Univ., ³2nd Dept.Pathol.,Fukusima Med.Coll.)

Osteopontin(OPN), RGD-containing glycoprotein has been suggested to function in adhesive interactions at the tumor-host interface and influence tumor invasion and metastasis. A monoclonal antibody was developed to synthetic peptide representing K170 through E187 sequences of human OPN,and used for ABC immunostaing. Formalin-fixed and paraffin-embedded human tumor tissues including 27 carcinomas(3 lung,1 tongue,2 esophagus,4 stomach,7 colon,9 breast,1 ovary),1 duodenal sarcoma,1 glioma,and 1 meningioma were examined. Macrophage-like cells were consistently positive in all tissues examined. In 16 tumors(53.3%),tumor cells in certain areas particularly necrosis and degenerating cells were positive. These results demonstrated OPN expression by macrophage-like cells and some tumor cells in formalin-fixed and paraffin-embedded human tumor tissues. A possible significance of OPN in tumor cells requires further study.