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Colon 26細胞が発現するCD44sは肝転移形成において細胞増殖制御に関与する：石井誠一, 原田伸彦, 斎藤一也, 溝井賢幸, 椎葉健一, 松野正紀（東北大・医・１外）

CD44s participates in the intrahepatic growth of murine colon 26 adenocarcinoma cells: Seiichi ISHII, Nobuhiko HARADA, Takayuki MIZOI, Kenichi SHIIBA, Kazuya SAITO, Seiki MATSUNO (First Dept. of Surg., Tohoku Univ. Sch. of Med.)

The purpose of this study was to determine if CD44, a metastasis-associated cell adhesion molecule, was involved in the hepatic colonization by murine colon 26 adenocarcinoma cells. Injections of 100,000 colon 26 cells into the superior mesenteric veins of syngenic BALB/c mice produced macroscopic hepatic nodules in 92% (22/24) of the mice on day 14. Colon 26 cells were strongly positive for surface CD44 and b1-Integrin determined by FACS analysis with monoclonal antibodies, IM7 and 9EG7, for CD44 and b1-Integrin, respectively. When colon 26 cells were pretreated with the IM7 antibody, only 30% (3/10) of mice developed hepatic metastasis on day 14 (P< 0.005) while the 9EG7 antibody did not significantly reduced metastasis formation. Histologically, microcolonies of tumor cells were observed in all of the livers on day 14 including the IM7-pretreatment mice that were free of gross nodules. RT-PCR amplification of mouse mRNA revealed that colon 26 cells and splenocytes only expressed the standard type of CD44 (CD44s) while normal murine colonocytes expressed possible variant isoforms. These data suggest that CD44s participates in the intrahepatic growth of colon 26 cells.