ポスターセッション

一覧　トップ

子宮体癌細胞由来の液性因子によるHL-60細胞の分化とThymidine phosphorylase (dThdPase) 誘導について：坂元秀樹^１,木下和哉^１,大谷香^１,中山靖夫^１,飯塚貞男²,佐藤和雄^１（^１日大・医・産婦,²東京都立広尾病院・産婦）

A novel humoral factor of endometrial carcinomas induce thymidine phosphorylase gene transcription and terminal differentiation in HL-60 cells: Hideki SAKAMOTO¹, Kazuya KINOSHITA¹, Kaori OHTANI¹, Yasuo NAKAYAMA¹, Sadao IIZUKA², Kazuo SATOH ¹ (¹Dept. OBGYN Nihon Univ. Scl. Med. ² Dept OBGYN, Tokyo Metropolitan Hiroo Hosp. )

dThdPase expression was studied in well differentiated endometrial carcinomas.　The expression was exclusively observed in the stromal macrophages (MΦ) of the cancerous portion of the endometrial stroma and not in benign lesions. The dThdPase expression co-localized with high neovascularization. These observation suggest that cancer may produce paracrine factor(s) to induce dThdPase in stromal MΦ and induce vascular formation by a dThdPase dependent manner. 40,000 HL-60 cells were cultured in (i) Nunc tissue culture inserts, placed in culture dishes of Ishikawa or mEIIL (a metastatic subclone of Ishikawa) endometrial cancer cells or with (ii) ascitic fluid from endometrial cancer or nonmalignant tumor bearing patients (n=10, 20) for 48 hours. Terminal differentiation was monitored by an attachment assay or phagocytotic phenomenon. The dThdPase induction was examined by (i) RT-PCR and (ii) Western blot (antibody kindly provided by Nippon Roche). HL-60 cells were negative for dThdPase mRNA whereas Ishikawa and mEIIL were both very weakly positive. HL-60 cells being exposed to the conditioned medium showed positive dThdPase. The exp- ression of dThdPase was approximately 1,000 fold higher in HL-60 cells exposed to mEIIL. Ascitic fluid from cancer patients showed concentration dependent induction of dThd Pase and differentiation of HL-60 cells. The characteristics of the factor will be discussed.