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慢性肝炎と分化型肝がんの肝細胞にみられたPKR過剰発現：嶋田明¹, 汐田剛史², 宮田博規¹, 釜洞俊雄¹, 川崎寛中², 日野茂男¹, 寺田忠史³ (鳥取大・医・¹ウイルス，²2内，³2病理)

Aberrant expression of double stranded RNA dependent protein kinase in hepatocytes of chronic hepatitis and differentiated hepatocellular carcinoma. Akira SHIMADA¹, Goshi SHIOTA², Hironori MIYATA,¹ Toshio KAMAHORA¹, Hironaka KAWASAKI², Shigeo HINO¹, Tadashi TERADA³ (Depts. Virology¹, 2nd Int. Med.², and 2nd Pathol.³, Facult. Med., Tottori Univ.)

Using a monoclonal antibody, 71/10, we immunohisto-chemically analyzed the PKR expression in 64 human liver biopsies and 25 liver sections of rats inoculated with diethylnitrosamine (DEN). The PKR expression in fatty and normal livers was minimum. Scoring signals from 0-4, the average scores of chronic 14-active (cah) and 6-persistent HCV-hepatitis (cph) were 2.8 and 2.0 (P = 0.038). For both cah and cph, the stained cells were more abundant in the periportal than in the centrilobular regions (P < 0.001). HCV liver cirrhosis also expressed PKR. The average scores of well-, moderately- and poorly-differentiated HCV-hepatocellular carcinomas (hcc) were 3.4, 2.1, and 0.3, respectively (P < 0.001 each). Those of well- and poorly-differentiated HBV hcc were 2.3 and 0.0, respectively. Those of rat hcc 5, 15, and 25 weeks after the last dose of DEN, 1.8, 2.0 and 2.0, were significantly higher than those of adenomatous hyperplasia, 0.9, 0.8 and 0.4, respectively. Most PKR-positive cells had integrated nuclei, and the PKR-index was approximately 20 and 100 times higher than the apoptotic index in HCV hepatitis and hcc, respectively.