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Study of Synergistic Apoptosis on Human Esophageal Carcinoma Cells Induced by the Combined Treatment of Irradiation and Hyperthermia : Takashi YUGUCHI, Mitsukazu SAITO, TomohiroSAITO, Yoshinobu YOKOYAMA, Toshihiro SAKAKIBARA, Katsunori TAUCHI, Tetsuro SHIMIZU, Takashi SAKAMOTO and Kazuhiro TSUKADA(Sec.Dept.of Surg.,Toyama Med.and Phar.Univ.)

Recently radiation therapy and hyperthermia have been employed as a part of the multimodal treatment of esophageal carcinoma and established that each of these modalities induces apoptosis in carcinoma cells. We examined the combined effect of these two modalities on an esophageal carcinoma cell line in vitro and investigated the participation of apoptosis.
The cells employed was SGF-8 cells which had been established from a human esophageal carcinoma. Cells were seeded in culture bottles, and after 24 h they were incubated at 43.5 ℃ for 60 min using a water bath or/and were irradiated at 2Gy / day for 5 days. On the 72 h culture following the treatments, the percent cell viability was determined by an MTT assay, the ratio of the cells apoptosis was analyzed using a flow cytometer in the single and combined groups.
In this study the combination treatment of hyperthermia and irradiation had a synergistic antiproliferative effect. Flow cytometric analysis revealed the synergistic increase of apoptotic cells in the combined treatment. In the cell cycle analysis, both hyperthermia and irradiation alone showed G2/M accumulation and this effect was more dominant in the combined group with the decrease of G1 and S-phase cells.