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食道扁平上皮癌におけるp27^Kip1の異常発現と腫瘍進展について：穴山貴嗣^1,2、降幡睦夫¹、松本　学^1,2、倉林　睦^1,2、園部　宏¹、大朏祐治¹、小越章平²（¹高知医大第二病理、²高知医大第二外）

Deregulated expression of p27^Kip1 protein associated with tumor progression in esophageal squamous cell carcinoma :
Takashi ANAYAMA^1,2, Mutsuo FURIHATA¹, Manabu MATSUMOTO^1,2, Atsushi KURABAYASHI^1,2, Hiroshi SONOBE¹, Yuji OHTSUKI¹, Shohei OGOSHI² (¹Dept. of Pathol. II, ²Dept. of Surg. II, Kochi Medical School)

p27^Kip1 (p27), one of the cyclin-dependent kinase (CDK) inhibitors (CDKIs), blocks progression from G1 to S phase by binding cyclin D1-CDK4 and/or cyclin E-CDK2. We immunohistochemically examined the relationships between p27, cyclin D1, cyclin E protein expressions and clinico-pathological factors in 77 patients with esophageal squamous cell carcinoma (SCC). Using p27, cyclin D1 and cyclin E protein antibodies, positive immunostaining in the nuclei was detected in 32.5% (25/77), 27.3% (21/77), and 29.6% (21/71) of patients, respectively. There were no statistically significant relationships among the expressions of these three proteins. Using Kaplan-Meier's method, each p27 and Cyclin D1 expression was found to be independently associated with a poor prognosis (p<0.05 and p<0.01). In multivariate analysis using the Cox-model, the expressions of p27 and Cyclin D1 retained predictive value for survival. In contrast to the former reports supporting a tumor-suppressive function of p27, the present study suggests that altered expression of p27 and cyclin D1 may be associated with the progression of human esophageal SCC, in which cyclin E may not play any key role. Further comprehensive analysis of p27, cyclin D1, cyclin E proteins by Western blotting was now under-investigation in order to elucidate immunopositivities.