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胃癌における抗アポトーシス遺伝子 "survivin" とテロメラーゼの発現：円谷彰，野口芳一，斉藤綾, 土井千春，岡本隆英，吉川貴己，福沢邦康，近藤治郎 (横浜市大, 医, 外)

An anti-apoptosis gene, survivin, and telomerase expression in gastric cancer: Akira TSUBURAYA, Yoshikazu NOGUCHI, Aya SAITO, Chiharu DOI, Takahide OKAMOTO, Takaki YOSHIKAWA, Kuniyasu FUKUZAWA, Jiro KONDO (Dept Surg, School Med, Yokohama City Univ)

Inhibition of programmed cell death (apoptosis) may play a significant role in cancer promotion and resistance to chemotherapy. Several inhibitors of apoptosis have been identified including oncogenes, bcl-2, and other biological factors. Survivin is recently identified as a novel human gene encoding a structurally unique IAP gene related to bcl-2. We investigated the expression of survivin and telomerase in human gastric cancer. Methods: Twenty-five consecutive patients with gastric cancer were studied. Tumors resected at operation were immediately freeze clamped and stored at -70℃. Total RNA of tumors was extracted for RT-PCR of survivin and telomerase (n=25) and DNA for a gel electrophoresis to evaluate fragmentation (n=15). Results: 1. Survivin mRNA was detected in 16 out of 25 gastric tumors and telomerase RNA in 24 out of 25 tumors. 2. Forty-four percent (4/9) of tumors with survivin mRNA and 67% (4/6) of these without survuvun exhibited DNA fragmentation. 3. DNA fragmentation was not found in the tumor without telomerase RNA, while in 14 telomerase positive tumors 8 (57%) had DNA fragmentation.Conclusion: Survivin may affect the biology of gastric cancer by inhibiting apoptosis independent with telomerase.