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マウス12番染色体上のアリル消失領域におけるがん抑制遺伝子の検討：朴永寛^1,2, 森展子¹, 宋昌祐³, 奥本正昭^1,2（大阪府立大・¹先端研・²農, ³韓国化学研）

Search for candidate tumor suppressor gene(s) in a frequent allelic loss region on mouse chromosome 12 : Yeong-gwan PARK^1,2, Nobuko MORI¹, Chang-woo SONG³, and Masaaki OKUMOTO^1,2 (¹Res. Inst. Adv. Sci. & Technol., ²Coll. Agric., Osaka Pref. Univ., ³Korea Res. Inst. Chem. Technol.)

We have observed frequent loss of heterozygosity(LOH) on chromosomes 4, 12 and 19 in radiation-induced lymphomas of (BALB/cHeA x STS/A)F1 hybrid mice, and also a high incidence of LOH on chromosome 12 in the lymphomas of (BALB/cHeA x MSM/Ms)F1 hybrid [(CXM)F1] mice. In this study we extensively investigated the highly frequent LOH region on chromosome 12 in 167 lymphomas from(CXM)F1 mice by PCR analysis using polymorphic microsatellite DNA markers. The highest frequency of LOH (109/167, 65.3%) was found at D12Mit233 locus. The LOH pattern of individual lymphomas indicates that the most common LOH region was between D12Mit53 (52 cM from the centromere) and D12Mit233 (52 cM)with syntenic homology to human chromosome 14q32.1. We then analyzed several genes which had been mapped around the precisely determined LOH region. Tumor necrosis factor-induced protein 2 (Tnfip2 or B94) and Igh genes were finely mapped telomeric to the LOH region. On the contrary, T-cell lymphoma breakpoint 1 (Tcl1) was located in the centromeric region. The results suggest that these genes were excluded from candidates responsible to the tumorigenesis. Analyses of other possibly tumor-related genes are in progress.