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L-[1-¹³C]phenylalanine 呼気テストによる、ラット肝部分切除後、肝再生率の測定：石井敬基¹,河野匡²,浅井聰³,伊藤あすか²,細井五三郎²,高橋泰夫¹,間崎武郎¹,藤井雅志¹,岩井重富¹,石川紘一³（¹日大医第3外,²東京ガス(株)フロンテイアテクノロジー研究所,³日大医薬理)

Determining liver regeneration rate in partially-hepatectomized rats, with the L-[1-¹³C]phenylalanine breath test: Yukimoto ISHII¹,Tadashi KOHNO², Satoshi ASAI³, Asuka ITO², Isaburou HOSOI², Yasuo TAKAHASHI¹, Takerou MAZAKI¹, Masashi FUJII¹, Shigetomi IWAI¹, Koichi ISHIKAWA³ (¹The 3rd Dept.of Surgery,³ Dept. of Pharmacology, Nihon University School of Medicine, and ²Frontier Technology Research Institute, Tokyo Gas, )

Aims: We intravenously administered L-[1-¹³C]phenylalanine to rats, and evaluated the relation between expiratory ¹³CO₂　and residual or regenerated liver weight after partial hepatectomy. Methods: The ¹³CO₂ excretion rate in expired gas, following intravenous administration of L-[1-¹³C]phenylalanine, was determined before and after partial hepatectomy, and the relations of the postoperative / preoperative ¹³CO₂ peak value to the postoperative residual liver weight / preoperative total liver weight and the regenerated liver weight / preoperative liver weight were evaluated. Results: The postoperative / preoperative ¹³CO₂ peak value % correlated with the postoperative residual liver weight / preoperative total liver weight % (r=0.949, p<0.0001) as well as the regenerated liver weight / preoperative total liver weight % (r=0.934, p<0.0001). Conclusion: We determined the expiratory ¹³CO₂ excretion rate using L-[1-¹³C]phenylalanine in rats with normal livers and found that the residual and regenerated liver weight percentages after partial hepatectomy can be measured based on the expiratory ¹³CO₂ peak value.